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My Engineering Work

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Post-Grad Research

Dynamic Regulation of the Chromatin Environment by ASH1L Modulates Human Neuronal Structure and Function (Ongoing)

The intersection of inflammation and DNA damage as a novel axis underlying the pathogenesis of autism spectrum disorders (Ongoing)

Mutations in ASH1L cause a neurodevelopmental disorder with sex differences in epilepsy and autism (Pre-Print)

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Undergraduate Research

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A Microfluidic Transfection Device to Modify Mammalian Cells (2023)

My senior capstone research project focused on developing a microfluidic device capable of delivering variable-sized payloads into 3T3 cells. We achieved high modification efficiency, repeatability, and scalability, with a 48-hour turnaround time. After iterating on the prototype, we submitted our design for a utility patent to allow subsequent research teams to continue our research. You can find my published work below (embargoed until April 2026).

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Hydraulic Modeling & Water Distribution Optimization in Kuna Nega, Panama (2022)

During the late Summer and early Fall of 2022, I had the great opportunity to travel to Panama City, Panama with a team of other students from WPI to work alongside IDAAN, the National Water Authority of Panama. For two months, we conducted field research regarding the state of the existing water delivery system that supplies the Kuna Nega District in the Greater Panama City Metropolitan Area. We modeled the system in an EPANET Simulation which we utilized to generate an optimized distribution schedule for the constituent neighborhoods, which maximized water availability while maintaining supply equity.

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3T3 Cell Population Density Limit and C2C12 Differentiation Independent Study (2021)

During the Fall and Winter of 2021, I worked with other students on some basic analyses of 3T3 (Mouse Fibroblast Cells) and C2C12 cells (Mouse Myoblast Cells). The focus of the work was, in part, to determine cell population doubling levels and the Hayflick's Limit of the 3T3 cells. The other portion of the work was to manually differentiate myoblast cells into myofibers.

During the 2019 - 2020 school year, as a team of four students, my group and I investigated various medical, cultural, and political causes that contribute to the high rates of obstetric fistula in young Bangladeshi women and proposed solutions based on the degree of social and economic feasibility.  We interviewed experts in the field who gave us first-hand accounts of life in the region and outlined aspects of life that helped us to develop a holistic approach to our solution.

A Study on the Minimization of Obstetric Fistula in Rangpur, Bangladesh (2020)

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My Creative Work

Get in Touch

Thanks for submitting!

stevenadefreitas@gmail.com

Biomedical Engineering | Cell Therapy | Prosthetics | Medicine

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